DTX1 drives ILT3 expression in CLL cells. (A) qRT-PCR analysis of transcription regulator expression in ILT3-positive and ILT3-negative MEC-pc clones (n = 6-8 clones). qRT-PCR analysis of DTX1/ILT3 expression (B) and flow cytometric analysis of surface ILT3 in control MEC cells, MEC cells with DTX1 CRISPR-mediated knockdown (MEC DTX1-KD), and MEC DTX1-KD cells transfected with either control vector or the same vector encoding DTX1 (C). Shown are mean values ± SEM. *P < .05. n.s., not significant.