ILT3 triggering inhibits BCR-dependent activation of Akt in CLL cells. Representative immunoblots (A) and relative quantitative analysis (B) of CLL cells stimulated for 3, 7, and 15 minutes with isotype control or ILT3-specific antibody in the presence or absence of BCR stimulation. Cells were isolated from the patients indicated in Table 2. Quantification analysis of phospho-Lyn (pLyn), pSyk, and pErk was performed for n = 12 patients; pAkt Thr308/Ser473 was analyzed for n = 17 patients. (C) Quantitative analysis of apoptotic annexin V–positive cells isolated from 4 CLL patients and stimulated for 24 hours as indicated. Shown are mean values ± SD. *P < .05; **P < .01; ***P < .001.