ILT3 inhibits Akt activation by recruiting active SHIP-1 to the BCR. (A) Representative immunofluorescence images and (B) colocalization analysis of phospho-SHIP-1 (Tyr1020) and surface ILT3 in BJAB-ILT3 cells stimulated as indicated for 15 minutes. (C) ILT3 immunoprecipitation (IP) from BJAB-ILT3-Streptag cells stimulated as indicated for 3 minutes. Left panel of immunoblots shows expression levels in the input lysates, and isolated protein complexes are shown on the right. (D) Representative immunofluorescence images and (E) colocalization analysis of phospho-SHIP-1 (Tyr1020) and surface BCR in BJAB-ILT3 cells stimulated as indicated for 15 minutes. Scale bars represent 5 μm. Shown are mean values ± SD. ***P < .001.