Survival curves of closely monitored clutches of zebrafish offspring from f10^+/− incrosses demonstrate progressive loss of 75% of homozygotes by 50 dpf and 100% by 115 dpf. There was no significant loss of heterozygotes (P > .05 by log-rank testing). Larvae were genotyped at 3 dpf, and selected individuals were observed daily. There was ∼20% background loss of individual fish across all genotypes up to 20 dpf, which is typical during wild-type fish development. See Figure 3B in the article by Hu et al that begins on page 666.