Distances from PD-L1⁺TAMs and PD-L1⁻TAMs to the nearest PD-L1⁺HRS cells. (A) Representative multiplex IF image (20× resolution; case P6) showing staining for CD30 (orange), CD68 (magenta), and PD-L1 (green). (B) Cellular phenotype map of the image shown in A depicting locations of PD-L1⁺ HRS cells (orange dots), PD-L1⁺ TAMs (purple dots), and PD-L1⁻ TAMs (pink dots). (C) Ray plot depicting the distance from each PD-L1⁺ TAM to the nearest PD-L1⁺ HRS cell. (D) Ray plot depicting the distance from each PD-L1⁻ TAM to the nearest PD-L1⁺ HRS cell. (E) The mean distances (microns) and standard errors for all 20 study tumors, divided into mean distance from PD-L1⁻ TAMs to the nearest PD-L1⁺ HRS cells (red) and mean distance from PD-L1⁺ TAMs to the nearest PD-L1⁺ HRS cells (blue). The tumors are ordered by the distances from PD-L1⁻ TAMs to PD-L1⁺ HRS cells, from highest to lowest. P value (<.0001) was calculated by paired Student t test. NN, nearest neighbor.