T-cell subsets in contact with TAMs. (A) Representative image (40× resolution; case N13) showing CD4⁺ T cells (left panel, green) with coexpression of PD-1 (right panel, yellow) touching CD68⁺ TAMs (left and right panels, magenta). (B) Membrane map depicting CD4⁺ T cells (PD-1⁺ dark green; PD-1⁻ light green), and PD-L1⁺ TAMs (purple). Cells are generally only outlined, with the exceptions of PD-1⁺ CD4⁺ T cells and PD-L1⁺ TAMs that are in contact, which are filled to highlight the interaction. (C) Representative image (40× resolution; case N13) showing CD8⁺ T cells (left panel, red) with coexpression of PD-1 (right panel, yellow) touching CD68⁺ TAMs (left and right panels, magenta). (D) Membrane map depicting CD8⁺ T cells (PD-1⁺ dark red; PD-1⁻ light red), and PD-L1⁺ TAMs (purple). Cells are generally only outlined, with the exceptions of PD-1⁺ CD8⁺ T cells and PD-L1⁺ TAMs that are in contact, which are filled. (E) Mean and standard error of the proportion of cells that are CD4⁺ T cells, CD8⁺ T cells, or undefined that are in contact with TAMs (red bars), within 75 µm of TAMs (blue bars), or more than 75 µm from TAMs (green bars), respectively. P values calculated by the Wilcoxon test. (F) Mean and standard error of the proportion of cells that are PD-1⁺ CD4⁺ T cells, PD-1⁻ CD4⁺ T cells, PD-1⁺ CD8⁺ T cells, or PD-1⁻ CD8⁺ T cells and that are in contact with PD-L1⁺ TAMs (red bars), within 75 µm of PD-L1⁺ TAMs (blue bars), or more than 75 µm from PD-L1⁺ TAMs (green bars), respectively. P values calculated by the Wilcoxon test.