ALL cell growth, viability, proliferation, and apoptosis are not regulated by VEGF. Analysis of cellular growth (cell numbers), proliferation (eFluor; decrease of label intensity on cell division), apoptosis (DNA fragmentation), and cellular viability (XTT; quantification of substrate turnover) of NALM-6 and KOPN-8 cells cultured in the presence of recombinant VEGF (A,C) or the VEGF-capturing antibody bevacizumab (beva) (B,D) and of NALM-6 cells with stable VEGF overexpression (E) or VEGF downregulation (F), showing VEGF-independent growth, proliferation, apoptosis, and metabolic activity of ALL cells. Graphs show results of 3 independent experiments, each run in triplicate; mean and standard error of the mean are shown. ctrl, control; GFP, green fluorescent protein; MFI, mean fluorescent intensity; miRNA, microRNA.