Figure 1.
Figure 1. Sensitivity of JAK2(V617F)+, CALR(del52)+, and MPL(ex10mut)+ cells to PARPi. (A) Cell lines expressing JAK2(V617F)+EpoR, CALR(del52)+MPL(wt), or MPL(W515L) were incubated with olaparib alone (1.25, 2.5, 5.0 μM) (squares) or BMN673 alone (12.5, 25.0, 50.0 nM) (squares), olaparib plus 200 μM vitamin E (circles), or olaparib plus 2.5 μM SCH51344 (triangles) for 96 hours in the presence of IL-3 plus Epo. Parental cells (diamonds) were incubated with olaparib or BMN673 only. Living cells were counted in Trypan blue. Results represent mean plus or minus SD percentage of living cells in comparison with untreated control from 3 independent experiments. (B) Western analysis of the indicated proteins in parental cells (P) and in isogenic cells expressing JAK2(V617F)+EpoR, CALR(del52)+MPL(wt), and MPL(W515L).

Sensitivity of JAK2(V617F)+, CALR(del52)+, andMPL(ex10mut)+cells to PARPi. (A) Cell lines expressing JAK2(V617F)+EpoR, CALR(del52)+MPL(wt), or MPL(W515L) were incubated with olaparib alone (1.25, 2.5, 5.0 μM) (squares) or BMN673 alone (12.5, 25.0, 50.0 nM) (squares), olaparib plus 200 μM vitamin E (circles), or olaparib plus 2.5 μM SCH51344 (triangles) for 96 hours in the presence of IL-3 plus Epo. Parental cells (diamonds) were incubated with olaparib or BMN673 only. Living cells were counted in Trypan blue. Results represent mean plus or minus SD percentage of living cells in comparison with untreated control from 3 independent experiments. (B) Western analysis of the indicated proteins in parental cells (P) and in isogenic cells expressing JAK2(V617F)+EpoR, CALR(del52)+MPL(wt), and MPL(W515L).

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