In silico studies of human Aα-chain amyloidogenic frameshift variants. (A) The TANGO aggregation scores of Phe521Leufs variant exhibited a very strong signal for β-sheet aggregation for VLITL at pH 2 (full line) and pH 8 (dotted line) at its C terminus. (B) PASTA2 predicted that VLITL is likely to stabilize the cross-β core of fibrillar aggregates and predicts parallel in-register intermolecular β-sheets for VLITL.