EGF regulates MCL-1 expression in TECs. (A) Histogram of MCL-1 expression in CD45⁻EpCAM⁺MHC II⁺ TECs from FTOC with or without 2-dGuo treatment. Histograms of MCL-1 expression in CD45⁻EpCAM⁺MHC II⁺ TECs from 2-dGuo-FTOC left untreated (blue) or stimulated (red) with RANKL, agonist CD40 antibody, and IL-22 (B); fibroblast growth factor 10 (FGF-10), hepatocyte growth factor (HGF), and EGF (C); EGF+U0126, EGF+PD98059, and EGF+PD0325901 (D). Data are representative of at least 2 experiments with n = 3/treatment. Numbers in panel D plots indicate the geometric mean of the fluorescence intensity. DMSO, dimethyl sulfoxide; IL-22, interleukin-22.