American Society of Hematology

Figure 3.

$Figure 3. IM did not interfere with the ACF-driven suppression of stem cell potential. Cells were treated with PBS or 5 µM ACF or 1 µM IM, alone or in combination (ACF+IM), and incubated at 0.1% O2 (LC1). (A) Viable cells were counted at day 3 of LC1. Data are expressed as fraction of the value obtained for PBS. Values represent mean ± S.D. of data obtained from 3 independent experiments; vs PBS: *P ≤ .01; vs ACF: #P ≤ .05; vs IM: §P ≤ .05. (B) Cells were transferred from day 7 LC1 to drug-free normoxic secondary cultures (LC2), to determine the maintenance of stem cell potential in LC1 via the counting of viable cells at the indicated times of incubation in LC2. Values represent mean ± S.D. of data obtained from 3 independent experiments.$

IM did not interfere with the ACF-driven suppression of stem cell potential. Cells were treated with PBS or 5 µM ACF or 1 µM IM, alone or in combination (ACF+IM), and incubated at 0.1% O₂ (LC1). (A) Viable cells were counted at day 3 of LC1. Data are expressed as fraction of the value obtained for PBS. Values represent mean ± S.D. of data obtained from 3 independent experiments; vs PBS: *P ≤ .01; vs ACF: #P ≤ .05; vs IM: §P ≤ .05. (B) Cells were transferred from day 7 LC1 to drug-free normoxic secondary cultures (LC2), to determine the maintenance of stem cell potential in LC1 via the counting of viable cells at the indicated times of incubation in LC2. Values represent mean ± S.D. of data obtained from 3 independent experiments.

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