ACF impaired serial colony formation and long-term culture-initiation abilities of primary CML cells. (A) Light-density BMMCs from CML patients were plated in methylcellulose-containing medium (I) and treated as indicated from time 0 of incubation (PBS, vehicle for ACF or IM). After 7 to 14 days, colony number was scored and cells replated in secondary, tertiary, or quaternary cultures (II, III, IV). Data are expressed as a fraction of the value obtained for PBS-treated cultures. Values represent mean ± S.D. of data obtained from experiments performed in duplicate; vs PBS: *P ≤ .05, **P ≤ .01; vs IM: #P ≤ .05, ##P ≤ .01; vs ACF: §P ≤ .05, §§P ≤ .01. (B) Light-density BMMCs from CML patients were treated as indicated, incubated at 0.1% O2 for 2 days and transferred to drug-free liquid cultures incubated at 21% O2. After 5 weeks, cells were replated in methylcellulose-containing medium and colony number was scored after 14 days. Data are expressed as fraction of the value obtained for PBS-treated cultures. Values represent mean ± S.D. of data obtained from experiments performed in duplicate; vs PBS: **P ≤ .01; ACF vs IM: ##P ≤ .01.
