Hematopoietic differentiation of marrow cells from healthy donors and patients. (A) Ordering of individual cells from 4 healthy donors (colored circles) into a 2-dimensional independent component space of hematopoietic lineages using Monocle. Lines connecting circles indicate edges of the minimum spanning tree (MST). A solid black line shows the main path of the MST, which provides a backbone for pseudotime ordering of cells by Monocol. ETP, earliest thymic progenitor; GMP, granulocyte-monocyte progenitor; MLP, multilymphoid progenitor; ProB, pro–B cell. (B-D) Mapping of patient cells onto normal MST by the nearest neighbor method (averaging locations of 5 nearest cells on the tree). Hollow circles indicate cells with monosomy 7 or del(7q) from patients 1, 2, 3, and 4 (patient 5 had trisomy 8).