Spatiotemporal expression of f10 in the developing zebrafish. (A) Phylogenetic tree of F10 from zebrafish (Danio), mouse (Mus), chicken (Gallus), and human (Homo). (B) RT-PCR from whole embryos demonstrates that zebrafish f10 mRNA is expressed during early embryonic development beginning at the 1-cell stage. Expression is relatively stable from 24 through 120 hpf. The expression of elongation factor 1-α (elfa) was used as an internal control. (C-L) WISH analysis of 120-hpf larvae shows that f10 expression is strong in the liver (D), but relatively weak in the yolk syncytial layer and brain (D-F), otic vesicles (E, G), and arches (H-I) by using an antisense probe (f10-as). (C) A sense control (f10-s) did not show any expression. (J-L) Plastic sections (5 µm) of stained larvae in transverse (J), sagittal (K), and coronal (L) planes are shown. Anterior is toward the left in panels C-H and toward the top in panel I. Scale bars (C-L), 100 µm. b, brain; ch, ceratohyal [2nd visceral pharyngeal arch (VA)]; li, liver; mc, mandibular cartilage (1st VA); ov, otic vesicle; sb, swim bladder; y, yolk; ysl, yolk syncytial layer.