Hdac8 deficiency results in altered hematopoietic output over time. (A) Western blot analysis of Hdac8 and β-actin in control (Ctrl) or Mx1-Cre/Hdac8^f/f(y) BM (13-15 months old) >1 year after poly (I:C) treatment. (B) Total number of BM cells harvested from Hdac8^Δ/Δ (n = 5) or Ctrl (Hdac8^f/f(y) ; n = 8) 1 year after poly (I:C) induction. (C) Frequency of lymphoid and myeloid lineage populations in Hdac8^Δ/Δ (n = 5) or Ctrl (n = 8) BM assessed by fluorescence-activated cell sorting (FACS). (D) Representative FACS plots showing gating strategy and frequency of phenotypic populations including LSKs, LT-HSCs, ST-HSCs, MPPs, GMPs, pre-GMs, pre-Meg/Es, and EPs from Hdac8^Δ/Δ or Ctrl BM. (E) Frequency of LSK, myeloid/erythroid progenitor populations in Hdac8^Δ/Δ (n = 5) or Ctrl (n = 8) BM. (F) Frequency of LT-HSC, ST-HSC, and MPP subsets in Hdac8^Δ/Δ (n = 5) or Ctrl (n = 8) BM. Lines indicate mean ± SEM. *P < .05, **P < .01, ***P < .001. MP, myeloid progenitor.