Figure 4.
Figure 4. Loss of Cks1 stabilizes p27kip1 in a proteasome-dependent manner. (A) Representative immunoblot analyses of p27kip1 expression in purified splenic pretumor B cells from 4-week-old mice after treatment with 25 μg/mL CHX with or without 40 μM MG-132 (MG) at indicated time points at 37°C. (B) The fold reduction of p27kip1 after CHX treatment was normalized to calnexin (a loading control), and the fold reduction of p27kip1 was calculated using cells at 0 hour of each genotype as the control. Combined data from 3 to 4 different mice represent the mean ± standard deviation. **P < .01, ****P < .0001 were calculated by 2-way ANOVA with multiple comparison tests.

Loss of Cks1 stabilizes p27kip1in a proteasome-dependent manner. (A) Representative immunoblot analyses of p27kip1 expression in purified splenic pretumor B cells from 4-week-old mice after treatment with 25 μg/mL CHX with or without 40 μM MG-132 (MG) at indicated time points at 37°C. (B) The fold reduction of p27kip1 after CHX treatment was normalized to calnexin (a loading control), and the fold reduction of p27kip1 was calculated using cells at 0 hour of each genotype as the control. Combined data from 3 to 4 different mice represent the mean ± standard deviation. **P < .01, ****P < .0001 were calculated by 2-way ANOVA with multiple comparison tests.

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