Expression of Il18 and Nlrc4 converge in barrier epithelial tissues. (A) RNA-seq expression values of Il1b and Il18 (left) and inflammasomopathy-associated genes (right) in murine immune cells derived from analysis of data from ImmGen project.⁶³  (B) The gcrma microarray expression values of Il18 and Il1b in diverse murine tissues from BioGPS dataset GNF1M.⁶⁴  Expression values of ex vivo LPS-stimulated macrophages are included as an internal high Il1b control. Microarray expression of (C) human immune cells or (D) tissues with consistent expression (Z > 5 in both duplicates) of IL1B or IL18. Data were derived from BioGPS dataset “Barcode on Normal Tissues.”⁶⁵  An Nlrc4 probeset was not present in multiple large murine tissue datasets, precluding a comparison of inflammasomes in human tissues. All graphs depict mean and standard error of the mean of all available replicates. Data for panels A-B were obtained from http://www.immgen.org and BioProject PRJNA2B1360. Data for panels B-D were obtained via http://www.biogps.org. DC, dendritic cell; GI, gastrointestinal; NKT, NK T cell; resp, respiratory; Treg, T regulatory cell.