Recombinant ERp72(ss-oo-oo) protein inhibits platelet accumulation and fibrin deposition in wild-type mice. Wild-type (WT) C57BL/6 mice were infused with ERp72 (ss-oo-oo) or bovine serum albumin (BSA; control) (150 μg per mouse), followed by cremaster arteriole injury. Platelets and fibrin were detected using anti-CD41 F(ab)2 conjugated to Alexa Fluor 488 and anti–fibrin antibody conjugated to Alexa Fluor 647. (A) Representative fluorescence images of intravital microscopy for platelet accumulation (green) and fibrin deposition (red). Original magnification ×64. Scale bars, 30 μm. Median fluorescent intensities (FIs) of anti–CD41 antibodies (platelets) (B) and anti–fibrin antibody (C) with the area under curve were analyzed using a Mann-Whitney rank-sum test; **P < .01; ***P < .001. The data were obtained from 30 thrombi in 3 mice for each experimental condition.