Recombinant ERp72(oo-ss-ss) protein directly enhances fibrin deposition. (A) Cremaster arteriole injury was induced in wild-type mice after they received intravenous injection of anti–fibrin antibodies conjugated to Alexa Fluor 647, with anti-CD41 F(ab)2 conjugated to Alexa Fluor 488 or 3′-dihexyloxacarbocyanine iodide (DIOC6) (2.5 μL of a 100 μM solution per gram of body weight). Representative images for platelet accumulation visualized by these 2 methods (green) and fibrin formation (red). The total original magnification is ×64. Scale bar, 30 μm. (B) Wild-type mice and β3^−/− mice received intravenous infusion of ERp72(oo-ss-ss) or BSA (control) (150 μg per mouse) as indicated, followed by cremaster arteriole injury. Platelets and fibrin formation were detected using DIOC6 and anti–fibrin antibody conjugated to Alexa Fluor 647. Median fluorescence intensities (FIs) of platelets (B) and fibrin (C) with the area under curve analyzed using a Kruskal-Wallis test. Only significant differences are shown; ***P < .001. The data were obtained from 40 thrombi in 5 mice for each experimental condition.