Reduced accumulation of AhR^−/−T cells in SLT. Donor cells were stained with Cell Proliferation Dye eFluor450 prior to transplant. 4.0 × 10⁶ WT or AhR^−/− T cells were transplanted with 3.0 × 10⁶ TCD BM cells into lethally irradiated B6D2 recipients. (A) Number of CD4⁺ donor T cells in SLT 4 days after transplant. (B) Frequency of proliferating cells CD4⁺ in SLT 4 days after transplant. Graphs represent pooled data from 2 independent experiments; n = 9 for each group. Flow cytometry plots show representative data of the ILN from 2 independent experiments. (C) Frequency of CD4⁺ cells expressing specific markers in the spleen 4 days after transplant; n = 4 in each group. Flow plots show representative histograms from the spleen. *P < .05; **P < .001 as indicated by Student t test.