Recipients of AhR^−/−T cells have increased numbers of pT_regcells in the colon. (A) Colons were isolated 14 days after transplant. Cells were gated on donor CD4⁺FoxP3⁺ T cells. These cells were analyzed for Neuropilin-1 (NRP-1) and Helios expression. Graph depicts pooled results from 2 independent experiments; n = 6 for WT, and n = 10 for AhR^−/− for each group. (B) Representative flow cytometry histograms of T-cell sorting for mRFP-negative cells. (C) 14 days after transplant, colons were isolated from recipients of either WT-FIR or AhR^−/−-FIR T cells and analyzed for mRFP expression. Flow cytometry panels are representative images of 3 independent experiments. Graphs represent pooled data of 3 individual experiments; n = 6 in each group. *P < .05 by Student t test. FITC, fluorescein isothiocyanate.