Regulated gene clusters in stimulated neutrophils. Neutrophils were cultured for 4 hours with N6/8-AHA (1 mM) (PKA), monocyte-conditioned supernatant (SN), GM-CSF (100 U/mL], LPS (1 μg/mL), and under hypoxic conditions, after which, the RNA was analyzed by Affymetrix microarray (GeneChip HU133 Plus 2.0 array). Using the DAVID Web-based functional analysis application we identified several functional terms that were overrepresented in the list of differentially expressed genes. This figure represents the differential expression status of genes in the Gene Ontology term GO:004281 (regulation of apoptosis), which was regulated by each stimulus. Greater than twofold upregulation is indicated in red, no change is indicated in gray, and greater than twofold downregulation is indicated in green. Shown are all genes (A) upregulated and (B) downregulated by N6/8-AHA. Please refer to supplemental Table 1 (available on the Blood Web site) for DAVID analysis files and supplemental Figure 1 for a complete figure of all regulated genes within GO:004281.