qPCR validation of selected targets identified from microarray data. Ultrapurified neutrophils were stimulated with N6/8-AHA (PKA) (1 mM), monocyte-conditioned SN, GM-CSF (100 U/mL), LPS (1 μg/mL), or cultured under hypoxic conditions for 4 hours. cDNA was prepared and qPCR performed for the following genes: (A) NR4A2, (B) NR4A3, (C) VEGFA, (D) IL-1α, and (E) IL-1β. Charts show mean ± SEM and are generated from 5 independent experiments. Statistical analysis was carried out by 1-way ANOVA and Dunnett’s posttest. Statistically significant comparisons are denoted by **P < .01 and ***P < .001, where treated populations were compared with control. RQ, relative quantity.