PGE2 promotes neutrophil survival and increases NR4A2/3 mRNA transcripts in the presence of LPS. Ultrapurified neutrophils were treated with 1 ng/mL LPS, 10 μM PGE2, or both LPS and PGE2 together for 18 hours. (A) Apoptosis was measured by light microscopy; data are expressed as means ± SEMs (n = 4). In parallel experiments, neutrophils were pretreated for 15 minutes with 0.7 mM Rp-8-Br-cAMPs (blue bars) or media (red bars) before the addition of LPS, PGE2, or both together, at the concentrations stated above for a further 4 hours. (B) NR4A2 and (C) NR4A3 expression was measured by qPCR. Charts show the fold change from media control, where NR4A expression was normalized to the GAPDH loading control; data are from 5 independent experiments. Statistical analysis was performed by 2-way ANOVA with Sidak’s posttests. Asterisks denote significant differences to the relevant media control. Pound symbols indicate differences between control and Rp-8-Br-cAMPS–treated conditions. Results were considered statistically significant at *^,#P < .05 and **P < .01.