CAM-DR is regulated by SOX11 through activation of the FAK/PI3K signaling pathway. (A) Z138CT/Z138KD (upper) and JVM2SOX11⁺/JVM2CT cells (lower), untreated (Ø) or pretreated with PF or IDEL, were seeded in coculture with stromal SNKT-GFP⁺ cells and incubated with BZM. After overnight coculture and BZM incubation, adherent MCL cells were labeled with Annexin V-PE and analyzed by FC. MCL cells were distinguished by GFP⁻ gating and cell size. Bar graphs correspond to the relative percentage of MCL cells cocultured with SNKTs that survived (Annexin V⁻ cells) relative to the corresponding SOX11⁺ untreated Annexin V⁻ MCL cells (Z138CTØ or JVM2SOX11⁺Ø, respectively). (B) SOX11⁺ MCL cells derived from PB (>95% purified) of primary MCLs (n = 5) untreated (Ø) or pretreated with PF and untreated SOX11⁻ (n = 4) MCL cells derived from primary MCL samples were seeded alone (−CC) or in coculture with stromal SNKT-GFP⁺ cells (+CC) and then incubated with BZM for 24 hours. The pool (supernatant and adhered) of MCL cells was labeled with Annexin V-PE and analyzed by FC. Bar graphs correspond to the relative percentage of MCL cell cocultured with SNKTs (+CC) that survived (Annexin V⁻ cells) relative to the corresponding Annexin V⁻ MCL cells in −CC. Bar plot represents the mean percentage ± standard deviation of 3 independent experiments. The significance of difference was determined by independent samples Student t test: *P < .05, **P < .01, ***P < .001.