Genome-wide microarray assay reveals differential expressed genes under IA conditions. C57BL/6 mice (N = 5) were fed a purified diet with no added iron and separated into ID, IA, and IO groups. Two weeks after iron injection, total hepatic RNA was subjected to Affymetrix GeneChip Mouse Gene 1.0 ST Array analysis to profile gene expression. (A) Cluster analysis of microarray showed different transcriptional profiles that were influenced by iron homeostasis. (B) Total hepatic RNA was subjected to qRT-PCR to determine HAMP, TFRC, FTL1, CCL2, and TNF-α expression. (C) Liver tissue was subjected to the immunofluorescent staining against CD68. Original magnification ×400.