An iron-rich diet leads to increased inflammatory responses and reduced HNF4α/miR-122 expression in hepatocytes. C57BL/6 mice (N = 6) were fed with either a standard diet or a 3% iron-rich diet for 1 month. (A-B) Serum ALT and AST levels (A) and hepatic inflammation scores (B) were determined. (C) H&E and immunofluorescent staining against CD68 showed inflammatory infiltration in the liver. Blue represents DAPI stain. Original magnification ×400. (D-E) mRNA (D) and protein (E) levels of various inflammatory factors and HNF4α were determined from isolated hepatocytes and macrophages. (F) Expression of miR-122 was determined. Data were normalized to the control group and are presented as mean ± SEM. *P < .05; **P < .01 vs control.