Silencing BACH2 in MCL increases cell proliferation and accelerates cell cycle progression. (A) Viable cells were counted using trypan blue staining in manipulated MCL cell lines. The data are presented the mean ± standard deviation (SD) from 3 independent experiments. Controls were mock-transfected cells (BACH2^Con). (B) Representative cell-cycle distribution of BACH2^KO or BACH2^Con MCL cells. (C) Representative data showing an intracellular pulse staining of BrdU in BACH2^KO or BACH2^Con MCL cells (left). The population of BACH2^KO cells in S phase was normalized to the control cells. Data are shown as the mean ± SD from 2 independent experiments (right). (D) mRNA levels of cell-cycle–related factors in BACH2^KO or BACH2^Con MCL cells. Each value from quantitative reverse-transcription PCR (qRT-PCR) was normalized to GAPDH and is presented as the mean ± SD. from 3 independent experiments. NS, not significant; *P < .05; **P < .01 (vs control cells; Student t test).