Effect of BCR surface density on tonic BCR signaling across GCB-DLBCL lines. (A) Correlation between BCR surface density based on CD79A-GFP fusion and reduction in proliferation caused by BCR KO in GCB-DLBCL lines. For reduction in proliferation, averages of 3 biological replicates (each with a different IgH-targeting gRNA) are displayed. (B) Correlation between BCR surface density based on CD79A-GFP fusion and BCR KO–induced reduction in AKT activity, as measured by percentage decrease of AKT activity reporter FRET efficiency, E, determined by FCM. For decrease of E, averages of 3 biological replicates (each with a different IgH-targeting gRNA) are displayed.