Figure 2.
Figure 2. Megakaryocytic output is reduced in P1 knock-in PreMegEs. (A) CFU-C activity of WT, P1-MRIPV/+, and P1-MRIPV/MRIPV PreMegE cells following culture in promyeloid methylcellulose medium (N = 4). (B-C) CD41 and Ter119 expression of OP9 cocultured PreMegE cells isolated on day 7. (B) Representative FACS plots; (C) quantification of CD41+ Mk and Ter119+ erythrocytes (N = 4). (D-E) Mk/Ery progenitor marker expression of PreMegEs following short-term (12 hours) culture in promyeloid liquid medium. (D) Representative FACS plots of PreMegE cells. (Top) CD150/CD41 expression of LK CD16/32− (LK 16/32−) progenitor cells. (Bottom) Endoglin/CD150 expression of LK CD41− CD16/32− (LK41− 16/32−) progenitors; (E) quantification of immunophenotypic PreMegEs, MkPs, PreCFUes, and CFUes relative to WT cultured cells (N = 4). *P < .05, **P < .01, ***P < .001, ****P < .0001. FSC-A, forward scatter–area.

Megakaryocytic output is reduced in P1 knock-in PreMegEs. (A) CFU-C activity of WT, P1-MRIPV/+, and P1-MRIPV/MRIPV PreMegE cells following culture in promyeloid methylcellulose medium (N = 4). (B-C) CD41 and Ter119 expression of OP9 cocultured PreMegE cells isolated on day 7. (B) Representative FACS plots; (C) quantification of CD41+ Mk and Ter119+ erythrocytes (N = 4). (D-E) Mk/Ery progenitor marker expression of PreMegEs following short-term (12 hours) culture in promyeloid liquid medium. (D) Representative FACS plots of PreMegE cells. (Top) CD150/CD41 expression of LK CD16/32− (LK 16/32−) progenitor cells. (Bottom) Endoglin/CD150 expression of LK CD41− CD16/32− (LK41− 16/32−) progenitors; (E) quantification of immunophenotypic PreMegEs, MkPs, PreCFUes, and CFUes relative to WT cultured cells (N = 4). *P < .05, **P < .01, ***P < .001, ****P < .0001. FSC-A, forward scatter–area.

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