The absence of RUNX1C results in the downregulation of pro-megakaryocyte and the upregulation of pro-apoptotic transcripts in PreMegEs. (A) Heat map depiction of genes at least 2-fold differentially expressed between WT and P1-MRIPV/MRIPV (MRIPV) PreMegE samples, as determined by RNA sequencing. Genes in blue are upregulated and genes in red are downregulated. (B) Signaling pathways repressed in MRIPV PreMegEs compared with WT PreMegEs as determined by IPA. (C-D) Reads per kilobase per million mapped reads (RPKM) values of selected Mk/Ery-associated Mk/Ery markers (C) and TFs (D). (E) Signaling pathways activated in KO PreMegEs compared with WT PreMegEs as determined by IPA. (F) RPKM values of selected apoptosis-associated factors. *P < .05, **P < .01, ***P < .001, ****P < .0001.