Figure 6.
Figure 6. RUNX1C is required for megakaryocyte progenitor proliferation and survival. (A) Representative FACS plots of DNA content (FxCycle Violet)/EdU incorporation in PreMegE (top), and MkP (bottom) populations. (B-C) Cell-cycle analysis of WT, P1-MRIPV/+, and P1-MRIPV/MRIPV PreMegE (B) and MkP (C) populations. Sub G0/G1: FxCycle Violet negative; G0/G1: EdU− 2N; S: EdU+ 2-4N; G2/M: EdU− 4N (N = 6). (D-G) Apoptosis analysis of MkPs. (D) Representative FACS plots of JC-1 green and red fluorescence in stained MkPs. (E) Quantitation of JC-1 red negative (apoptotic) MkPs. (N = 4) (F) Representative FACS plots of 7-AAD/Annexin V (AnnV) staining of MkPs. (G) Quantitation of AnnV+ 7-AAD− (early apoptotic), AnnV+ 7-AAD+ (late apoptotic/dead) MkPs (N = 3). *P < .05, **P < .01, ***P < .001, ****P < .0001. EdU, 5-ethynyl-2′-deoxyuridine.

RUNX1C is required for megakaryocyte progenitor proliferation and survival. (A) Representative FACS plots of DNA content (FxCycle Violet)/EdU incorporation in PreMegE (top), and MkP (bottom) populations. (B-C) Cell-cycle analysis of WT, P1-MRIPV/+, and P1-MRIPV/MRIPV PreMegE (B) and MkP (C) populations. Sub G0/G1: FxCycle Violet negative; G0/G1: EdU− 2N; S: EdU+ 2-4N; G2/M: EdU− 4N (N = 6). (D-G) Apoptosis analysis of MkPs. (D) Representative FACS plots of JC-1 green and red fluorescence in stained MkPs. (E) Quantitation of JC-1 red negative (apoptotic) MkPs. (N = 4) (F) Representative FACS plots of 7-AAD/Annexin V (AnnV) staining of MkPs. (G) Quantitation of AnnV+ 7-AAD− (early apoptotic), AnnV+ 7-AAD+ (late apoptotic/dead) MkPs (N = 3). *P < .05, **P < .01, ***P < .001, ****P < .0001. EdU, 5-ethynyl-2′-deoxyuridine.

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