TEC-specific deletion of p53 affects the establishment of peripheral T-cell tolerance. (A) Representative analysis (H&E staining) of the salivary and lacrimal glands obtained from 6- to 7-month-old Ctr and p53cKO mice. (B) Histological scores of inflammatory infiltrates. Pie graphs show the frequency of inflammatory lesions according to their severity. (C) Thymocytes from 10-week-old Ctr (red line) and p53cKO (blue line) mice were intravenously transferred into Rag2^−/− mice. As a control, Rag2^−/− mice were treated with phosphate-buffered saline (green line). Change in the body weight of Rag2^−/− recipients after thymocyte transfer (left). Incidence of diarrhea and weight loss, with the indicated number of recipient mice that develop symptoms (middle). Images show the histological analysis (H&E staining) of the colon of the Rag2^−/− recipients 14 days after transfer (right). (D) Histological analysis and inflammatory score of the salivary glands. Pie graphs represent the frequency of inflammatory lesions according to their severity. Arrows in panels A, C, and D outline lymphocytic infiltrates. Results are presented as mean ± SEM. Scale bars, 100 μm. PBS, phosphate-buffered saline. *P < .05; **P < .01; ***P < .001.