Characterization of hematopoietic cells accumulating in the AdCre/KRAS^G12Dlung. (A) Histological analysis of the AdCre/KRAS^G12D lung. Top, low magnification images of H&E-stained lung sections from AdCre/KRAS^WT and AdCre/KRAS^G12D mice at 4 months following nasal delivery of AdCre. Scale bar, 0.5 mm. Middle and bottom, high magnification images of H&E staining (middle) and S100 IHC (bottom), corresponding to the areas indicated in the top right image of the AdCre/KRAS^G12D lung (left, solid mass lesion; right, DIP-like alveolar lesion). Scale bar, 62.5 μm. (B) Flow cytometry analysis of hematopoietic compartments in the AdCre/KRAS^G12D lung in comparison with AdCre/KRAS^WT controls. Representative contour plots for CD11b-F4/80 and CD11b-CD11c staining are indicated in the left, and the right bar graph summarizes the percentage of each hematopoietic population in total lung cells. The distribution of CD11b^lowF4/80⁺ (blue) and CD11b^int-highF4/80⁺ (green) cells are indicated in CD11b-CD11c plots in the bottom left. The data in the right bar graph represent mean + standard deviation (SD) (n = 3). WT, wild type.