KRAS^G12D-induced aberrant growth of lung CD11b^intCD11c^dimGr1⁻cells. (A) Flow cytometry profiling of lung myeloid cells in noninduced KRAS^LSL-G12D mice. CD45⁺ gated lung hematopoietic cells contain CD11b^lowCD11c⁺ (Fr-1), CD11b^intCD11c^dimGr1⁻ (Fr-2), CD11b^intCD11c-Gr1^int (Fr-3), and CD11b^highGr1^high (Fr-4) myeloid populations. Gr1 expression profiles of the 4 myeloid populations are indicated in the bottom CD11c/Gr1 contour plot. (B) PCR detection of recombination of the KRAS^G12D allele in the sorted myeloid populations (Fr-1 to 4 from noninduced KRAS^LSL-G12D mice) infected with AdCMVCre or AdCMVβgal at MOI = 100 for 72 hours. (C) Representative phase-contrast images of AdCMVβgal or AdCMVCre-infected Fr-1/2 cells (sorted from noninduced KRAS^LSL-G12D mice) cultured on lung fibroblast feeders for 4 weeks. Original magnification ×200. (D) Flow cytometry analysis of noninduced Fr-1/2 cells for DC marker expression.