Analysis of thrombus formation in vivo. (A) Photochemical-induced arterial thrombosis assessed as time required for occlusion of femoral artery measured by laser Doppler in WT (red bars) and APP-KO (blue bars) mice. Data are the mean ± SEM of measurements performed on 5 animals for both genotypes. (B) Analysis of deep vein thrombosis. Thrombus formation in the IVC was induced by vein ligation. Thrombi were extracted 24 or 48 hours after surgery. (i) Representative image showing the different size of thrombi from WT and APP-KO mice after 24 hours of ligation. Quantifications of thrombus length and weight at 24 and 48 hours are reported in (ii) and (iii), respectively. **P < .01; ***P < .005. (C) Analysis of thrombus embolization. (i) Staining of lung sections from WT or APP-KO with phosphotungstic acid–hematoxylin. Samples were prepared 48 hours on IVC ligation. Fibrin-occluded vessels are indicated by the arrows. (ii) Analysis of the percentage of occluded vessel observed in the 2 genotypes. Data are presented as mean ± SEM. n = 5. **P < .01. (D) Comparison of venous thrombosis in WT mice transplanted with bone marrow cells from WT animals (WT/WT) and in WT mice transplanted with bone marrow cells from APP-KO mice (WT/APPKO). Thrombus length is reported in (i) and thrombus weight in (ii). *P < .05; **P < .001.