PS and TFPI in human synovium. (A) PS and TFPI are expressed in synovial tissue of patients with HA (on demand and on prophylaxis), HB on demand or osteoarthritis (OA). Arrowheads point to synovial lining layer and arrows to vascular structures in the sublining layer, all positive for both PS and TFPI. Scale bars: 50 µm. (B) Western blot analysis of conditioned media of primary human FLS cultures from a healthy individual and an OA patient before and after deglycosylation using anti-TFPI antibody. Human platelet lysate was used as a positive control for TFPIα. Blots are representative of 3 independent experiments. DEGL., deglycosylation; hFLS, human FLS; hPLT, human platelet lysate; OA, osteoarthritis.