Discordant HTS and FC MRD have an intermediate prognosis. A Kaplan-Meier estimate of EFS between patients who were negative for MRD as measured by both HTS and FC, positive for HTS and FC, and HTS positive/FC negative at a threshold of 0.01% identified a cohort of 55 patients who were negative for MRD by FC and positive by HTS. These patients had an intermediate prognosis between patients who were negative by both HTS and Flow and patients who were positive by both HTS and Flow. For the Flow-negative group, the outcome was worse for patients who were HTS positive (P = .036).