Transcriptomic alterations in EBV⁺-DLBL from PDX models. (A) Increased Rho pathway signature in EBV⁺-DLBL from PDX models. Alterations of the Rho pathway were identified by pathway analysis using 3 tumor types (EBV⁺-DLBLs, gastric adenocarcinomas, and DLBLs from the TCGA database). (B) EBV gene expression activity across the entire EBV genome. The EBV viral gene activation pattern differed between EBV⁺-DLBLs from our study (X102 is representative) and EBV-associated gastric adenocarcinomas from the TCGA database (TCGA BR-4253-01A is representative). (C) Expression profiles of latency and lytic cycle-related EBV genes. EBV⁺-DLBL showed a latency III pattern in gene expression, whereas limited latency-related gene expression was present in EBV⁺ gastric adenocarcinoma. Lytic cycle-related genes were expressed in all 3 lytic cycle stages in EBV⁺-DLBLs, but gene expression was limited in TCGA adenocarcinoma. (D) Correlation between the EBV latency III-related gene signature and Rho pathway signature. Each dot represents an EBV⁺-DLBL sample, and the numbers in the graph indicate P-value and Pearson correlation coefficient (R) of the correlation.