Differentiation potentials of cCLPs in vivo. CD45.1⁺cCLP cultured for 2 months were transplanted into sublethally irradiated CD45.2⁺C57BL/6 mice (n = 4). Bone marrow (top), spleen (middle), and thymus (bottom) of the recipient hosts were FACS analyzed at the indicated times after transplantation. (A) Total numbers of CD45.1⁺ donor cells. (B) Representative FACS profiles of donor-derived T and B cells differentiated at 3 weeks after transplantation in bone marrow (upper panel), spleen (middle panel), and thymus (lower panel). (C-D) Total numbers of indicated cell subsets derived from donors in bone marrow (C) and spleen (D) at different times after transplantation. (E) Representative FACS profiles of donor-derived NK1.1⁺NK cells (upper panel), CD11b⁺Gr1⁺ myeloid cells (middle panel) in bone marrow, and CD11b⁺CD11c⁺, CD11b⁻CD11c⁺ DCs in spleen (lower panel) at different times after transplantation. The values in each panel shown in panels B and E indicate the percentages of cells in each gate. Data shown in panels A to E are representative of 2 independent experiments. (F) Representative FACS profiles and (G) total numbers of donor-derived ILC subsets in intestine 4 weeks after transplantation of CD45.1⁺ cCLPs cultured for 2 months into sublethally irradiated CD45.2⁺ RAG2/c-deficient mice (n = 4). BM, bone marrow; IgM, immunoglobulin M; Spl, spleen; Thy, thymus.