Macrophages and mast cells respond to EV treatment. PKH67-labeled EV_MWCL (yellow) were added to iBMDMs (A) and BMCMCs (D) to observe EV internalization. Bar, 10 μm. iBMDMs (B-C) were seeded and stimulated with EV_CONT, EV_L265P (30 μg/mL), or EV_MWCL, EV_BCWM, and EV_Ramos as negative control (100 μg/mL) and LPS as positive control (10 ng/mL) for 16 hours. BMCMCs (E-F) were seeded and stimulated with EV_CONT, EV_L265P (30 μg/mL), or EV_MWCL and EV_Ramos as negative control (100 μg/mL) and LPS as positive control (50 ng/mL) for 16 hours. RNA was isolated and qPCRs for Rantes and Il6 were performed.