IL4R-I242N induced tumorigenesis in murine xenografts. (A) Tumor volume and (B) Kaplan-Meier survival curves from NSG mice xenografted with DEV cells expressing IL4R^WT (n = 6) or IL4R^I242N (n = 5). (C) mRNA expression of IL4R, CCL17, and CD23 measured by quantitative real-time PCR in xenograft tumors. (D-E) Ki67 and H&E staining performed in xenograft tumors. (D) Representative IHC images are shown and the (E) percentage of Ki67⁺ cells was measured using automatic cell counting with ImageJ. Images were acquired using Eclipse E600 microscope, DS-F1 camera, and DS-L2 acquisition software (Nikon); original magnification ×40; numerical aperture of objective lenses, 0.75. Graphs show the mean ± SD and significance evaluated using 2-way ANOVA with (A) Bonferroni posttest and (C,E) 2-sample unpaired 2-tailed Student t test. *P < .05; **P < .01; ***P < .001; ****P < .0001.