American Society of Hematology

Figure 1.

$Figure 1. Myo1f was dispensable for leukocyte rolling and adhesion but essential for neutrophil extravasation in inflamed mouse cremaster muscle venules. (A-C) Intravital microscopy of postcapillary venules in mouse cremaster muscle 2.5 hours after intrascrotal injection of TNFα (500 ng per animal). (A) Rolling flux fraction, (B) rolling velocity, and (C) number of adherent leukocytes (n = 15 venules of 4 Myo1f+/+ mice and n = 12 venules of 3 Myo1f−/− mice). (D) Rheological parameters of cremaster muscle venules after TNFα injection. (E) Representative images (left panel) of postcapillary venules of cremaster muscle whole mounts stained with Giemsa’s azur eosin methylene blue. Scale bar, 20 µm. Quantification of perivascular neutrophils and other leukocyte subtypes (others, right panel) (n = 18 vessels of 3 Myo1f+/+ mice and n = 17 vessels of 3 Myo1f−/− mice). Mean ± SEM; *P < .05 (2-way ANOVA, Sidak multiple comparison test). n.s., not significant; WBC, white blood cell.$

Myo1f was dispensable for leukocyte rolling and adhesion but essential for neutrophil extravasation in inflamed mouse cremaster muscle venules. (A-C) Intravital microscopy of postcapillary venules in mouse cremaster muscle 2.5 hours after intrascrotal injection of TNFα (500 ng per animal). (A) Rolling flux fraction, (B) rolling velocity, and (C) number of adherent leukocytes (n = 15 venules of 4 Myo1f^+/+ mice and n = 12 venules of 3 Myo1f^−/− mice). (D) Rheological parameters of cremaster muscle venules after TNFα injection. (E) Representative images (left panel) of postcapillary venules of cremaster muscle whole mounts stained with Giemsa’s azur eosin methylene blue. Scale bar, 20 µm. Quantification of perivascular neutrophils and other leukocyte subtypes (others, right panel) (n = 18 vessels of 3 Myo1f^+/+ mice and n = 17 vessels of 3 Myo1f^−/− mice). Mean ± SEM; *P < .05 (2-way ANOVA, Sidak multiple comparison test). n.s., not significant; WBC, white blood cell.

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