The expression of transduced TCRs in primary CD8⁺ αβ T cells derived from 3 healthy donor PBMCs is markedly increased in the presence of CRISPR/Cas9 specific for endogenous TCR-β. Histograms represent staining of transduced CD8⁺ cells with a pan-γδ TCR antibody (A) or with a HLA-A2:EAAGIGILTV tetramer cognate for Mel13 TCR (B), whereas the numbers refer to geometric mean intensity of staining. Black indicates unstained control; gray, untransduced T cells; blue, transduced only with a TCR; red, transduced with a TCR and CRISPR.