SnPP injections rescue anemia and reduce spleen size, reticulocyte levels, serum iron concentration, transferrin saturation, and EPO levels in Th3/⁺mice. (A) RBC, (B) Hb, (C) HCT, (D) MCV, and (E) MCH indices were determined on Wt + PBS (n = 12), Wt + SnPP (n = 12), Th3/⁺ + PBS (n = 11), and Th3/⁺ + SnPP (n = 12) mouse groups by automated analysis. (F) Representative images of spleens isolated from Wt and Th3/⁺ mice injected either with PBS or SnPP. (G) Spleen weight index of Wt + PBS (n = 9), Wt + SnPP (n = 9), Th3/⁺ + PBS (n = 8), and Th3/⁺ + SnPP (n = 7) mice. (H) Flow cytometry analysis of thiazole orange staining of peripheral blood obtained from Wt and Th3/⁺ mice injected either with PBS or SnPP (n = 6). Analysis of (I) serum iron and (J) transferrin saturation in Wt + PBS (n = 5), Wt + SnPP (n = 5), Th3/⁺ + PBS (n = 5), and Th3/⁺ + SnPP (n = 4) mice. (L) Enzyme-linked immunosorbent assay of EPO in serum from Wt and Th3/⁺ mice injected with PBS or SnPP (n = 11). *P < .05, ***P < .001.