Under physiological flow conditions (1 dyne/cm2), murine neutrophils attach to membranes coated with recombinant murine ICAM-1 (rmICAM-1) and rmP-selectin and successfully transmigrate through pores into collagen gel containing a chemoattractant molecule. In contrast, Myo1f-deficient neutrophils are mostly unable to transmigrate, and the majority of neutrophils are found within the flow chamber or stuck within the pores. This figure provides a robust demonstration of the need for Myo1f for neutrophils to migrate through 3D environments in vitro and an explanation for the failure of Myo1f-deficient neutrophils to accumulate into sites of inflammation in vivo. See the complete Figure 3 in the article by Salvermoser et al that begins on page 1887.
