Reduced number of B cells in peripheral tissues of Eμ/miR-125b-Tg mice. (A) Frequency of B220⁺ B cells in peripheral tissues from 8-week-old Eμ/miR-125b-Tg and control mice. Lymphocytes from the bone marrow, peripheral blood, and spleen and PLNs from control and Eμ/miR-125b-Tg mice were analyzed by flow cytometry. (B) Total number of leukocytes (CD45⁺) and B cells (B220⁺), T cells (CD4⁺ or CD8⁺), or myeloid cells (CD11b⁺) counted in each organ or per microliter of peripheral blood. Bars represent mean values of pooled data. Data are pooled from 2 to 3 experiments and represented as mean ± SEM (n = 10-12 mice per group). LN, lymph node. *P < .05; **P < .01; ***P < .001; NS, not significant.