Serum ferritin levels are affected in mice with trafficking defects of the endolysosomal pathways. (A) Serum ferritin levels were estimated by enzyme-linked immunosorbent assay. (B) Serum iron and all other blood tests were performed by trained staff in a veterinary laboratory. (C) Transferrin saturation percentage was calculated by dividing serum iron by total iron-binding capacity and multiplying by 100. (D) Liver iron was evaluated using a colorimetric ferrozine-based assay. (E) Transferrin receptor 1 (TfR1) western blot. Liver lysates were separated by SDS-PAGE (35 µg protein/lane) and subjected to western blotting with anti-TfR1 and antiactin antibodies. Representative results in livers from 2 out of 4 mice are shown. Measurements of BLOC-1^−/−, BLOC-2^−/−, and BLOC-3^−/− mice were compared with those of WT C57BL/6J mice. Rab27A^−/− mice were on a C3H background and were compared with their WT counterparts. Each bar represents mean ± SD (*P < .05, **P < .01, ***P < .001). Statistical significance was evaluated by an unpaired t test using GraphPad software.