Figure 2.
Influence of H2O2modulation on BCR signaling. (A) CLL cell samples were stimulated with 10 µg/mL anti-IgM for 10 minutes in the absence or presence of 3.3 mM H2O2, and signaling response was measured as the log2-fold change of ERK1/2 phosphorylation between modulated and unmodulated conditions. Samples were grouped in BCR-nonresponder and BCR-responder, referring to the median value of ERK1/2-response to anti-IgM. Comparison of signaling responses was performed using the Student t test. Lines indicate the mean value. ***P ≤ .001. (B) Representative contour plots of pERK1/2 in unmodulated condition or after modulation with anti-IgM, in the absence or presence of H2O2.
