Association of redox sensitivity with ROS levels, mitochondrial amounts, and NADPH-oxidase. Patients were grouped on the basis of high and low H2O2-sensitive values, referred to the median signal values of the combined sum of pSYK, pERK1/2, and pp38 responses (each measured as log2-fold change). Comparisons between the high and low H2O2-sensitive groups for (A) total cell ROS levels (detected by 2′,7′-dichlorodihydrofluorescein diacetate probe; n = 18), (B) mitochondrial-specific ROS levels (detected by MitoSOX probe; n = 17), (C) mitochondrial amounts (detected by MitoTracker probe; n = 18), and (D) relative gene expression of the gp91phox NOX subunit (n = 26) were performed using the Student t test. Data are expressed as mean ± SEM. *P ≤ .05.
